Experimental Techniques in Study of Metabolic and Endocrine Disorders

Karolinska Institutet

This course will enable doctoral students and postdocs to acquire the necessary knowledge to address experimentally keypoints of metabolic characterization of experimental models in diabetes research.

The course is laboratory based, aiming to give all participants hands on experience with isolated pancreatic islets, skeletal muscle and adipose tissue. Techniques for measurement of glucose transport in isolated rodent skeletal muscle, of lipolysis in isolated adipocytes, and for studying insulin release from pancreatic islets will be covered.

Theoretical and practical considerations will be presented and discussed.

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